TFM2_18S_amplicons_analysis
lrubinat
3/9/2017
1) 18S amplicons
1.1) Data overview
The dataset “Malaspina 18S iTags” contains reads from 122 samples of Malaspina. On average, each sample contains 126100 reads:
## Min. 1st Qu. Median Mean 3rd Qu. Max.
## 4050 59560 98630 126100 157000 935800
Overall reads per sample:
1.2) Normalization
In order to keep as many samples as possible, we rarefy at 49975 reads per sample. By that, we loose 31 samples, and after removing the exluded samples in the 16S dataset (to make them comparable), we end up with a normalized dataset containing 43 samples and 38338 OTUs.
Datasets summary:
dim(tb18_tax) #original dataset## [1] 46690 129dim(tb18_tax_occur) #original dataset with occurrence data alone## [1] 46690 122dim(tb18_tax_occur_min49975) #dataset without samples with less than 49975 OTUs## [1] 43734 91dim(tb18_tax_occur_ss49975_no_cero) #rarefied dataset## [1] 91 383381.3) General community analysis
1.3.1) Richness and evenness (Shannon index)
Most of the samples take Shannon Index values around 6:
1.3.2) Richness: OTU number
Lowest number of OTUs per sample:
## [1] 1248Maximum number of OTUs per sample:
## [1] 5183In most of the samples, we can identify about 4000 OTUs:
1.3.3) Index of evenness
1.3.3.1) Pielou’s index
The Pielou index (constrained between 0 and 1) takes values closer to 1 as the variation of species proportion in a sample increases. Most of the samples get values between 0.7 and 0.8, meaning that the numerical composition of different OTUs in a sample is variable - there’s no constant presence of dominant species.
1.3.4) Abundance Models
Most of the OTUs show very few occurrences; suggesting that we will probably be able to identify a significant ammount of rare otus:
1.3.4.1) Rank-Abundance or Dominance/Diversity Model (“radfit”)
The OTUs abundance distribution fits relativelly close to log-normal model.
1.3.4.2) Preston’s Lognormal Model
According to Preston’s lognormal model fit into species frequencies groups, we’re missing ~2606 species:
## Extrapolated Observed Veiled
## 46340.804 43734.000 2606.804
When computing Prestons’ lognormal model fit without pooling data into groups, we miss ~2463 species:
## Extrapolated Observed Veiled
## 46197.162 43734.000 2463.1621.3.5) Rarefaction curves of rarefied and non-rarefied datasets
1.3.6) Beta diversity
1.3.6.1) Dissimilarity matrix using Bray-Curtis index:
The Bray-Curtis dissimilarity, constrained between 0 (minimum distance) and 1 (highest dissimilarity) allows us to quantify the differences between samples according to the composition and relative abundance of their OTUs. In our dataset, most of the samples pairs take dissimilarity values between between 6.5 and 7.5, meaning that their composition is different.
1.3.6.2) Hierarchical clustering
The stations seem to form clusters according to geographic localization, but there are no evident clusters separated from the general groups.
(To be done: assign Longhurst provinces information to each station and check if any of the central clusters is meaningful regarding to the samples’ geographical location)
1.3.6.3) Non-metric multidimensional scaling
We can only identify a prominent group in the central part of the NMDS plot. The stress parameter takes a value below 0.2, suggesting that the plot is acceptable.
##
## Call:
## monoMDS(dist = tb18_tax_occur_ss49975_no_cero.bray)
##
## Non-metric Multidimensional Scaling
##
## 91 points, dissimilarity 'bray', call 'vegdist(x = tb18_tax_occur_ss49975_no_cero, method = "bray")'
##
## Dimensions: 2
## Stress: 0.2108913
## Stress type 1, weak ties
## Scores scaled to unit root mean square, rotated to principal components
## Stopped after 86 iterations: Stress nearly unchanged (ratio > sratmax)
1.4) Geographical analysis
Communities quickly change their composition across geographical distances:
1.4.1) Mantel correlograms
Mantel statistic is -significantlly- so low, meaning that the correlation between samples dissimilarity and geographical distances is weak.
##
## Mantel statistic based on Pearson's product-moment correlation
##
## Call:
## mantel(xdis = geo_distances_MP_18S, ydis = tb18_tax_occur_ss49975_no_cero.bray)
##
## Mantel statistic r: 0.1713
## Significance: 0.001
##
## Upper quantiles of permutations (null model):
## 90% 95% 97.5% 99%
## 0.0196 0.0259 0.0315 0.0360
## Permutation: free
## Number of permutations: 999Correlograms:
MP_18s_ss49975_mantel_correl_by_1000km<-mantel.correlog(tb18_tax_occur_ss49975_no_cero.bray, D.geo=geo_distances_MP_18S, break.pts=seq(0,20000, by=1000))
plot(MP_18s_ss49975_mantel_correl_by_1000km)
MP_18s_ss49975_mantel_correl_by_100km<-mantel.correlog(tb18_tax_occur_ss49975_no_cero.bray, D.geo=geo_distances_MP_18S, break.pts=seq(0,20000, by=100))
plot(MP_18s_ss49975_mantel_correl_by_100km)
1.5) Abundance vs. occurence
OTUs distribution according to their percentage of occurence and relative abundance.
- red line: OTUs that occur in more than 80% of the samples.
- blue line: regionally abundant OTUs (> 0.1%).
- green line: regionally rare OTUs (< 0.001%).
Regionally abundant OTUs (relative abundance over 0.1%):
## otu_names mean_rabund perc_occur SILVA_plus_MAS_plus_BM_classif
## 125 OTU_6 0.037234661 100.00000 <NA>
## 73 OTU_2 0.035301387 100.00000 <NA>
## 78 OTU_23 0.012482065 96.70330 <NA>
## 148 OTU_8 0.011335998 98.90110 <NA>
## 82 OTU_25 0.010648841 100.00000 Dinophyceae
## 84 OTU_27 0.010030730 100.00000 <NA>
## 1 OTU_1 0.008782193 100.00000 Dinophyceae
## 27 OTU_12 0.008684342 100.00000 <NA>
## 136 OTU_7 0.007983332 100.00000 <NA>
## 159 OTU_9 0.007803902 73.62637 <NA>
## 74 OTU_20 0.007539154 56.04396 Pelagophyceae
## 102 OTU_4 0.007484402 100.00000 Dinophyceae
## 37 OTU_13 0.007211298 98.90110 <NA>
## 14 OTU_11 0.007044621 98.90110 <NA>
## 52 OTU_14696 0.006932257 95.60440 <NA>
## 65 OTU_18 0.006813077 100.00000 Dinophyceae
## 80 OTU_24 0.006641343 98.90110 <NA>
## 87 OTU_3 0.005921862 85.71429 <NA>
## 93 OTU_34 0.005863591 71.42857 Chrysophyceae
## 75 OTU_21 0.005359603 94.50549 <NA>
## 83 OTU_26 0.005116624 95.60440 <NA>
## 113 OTU_5 0.004815375 80.21978 <NA>
## 76 OTU_22 0.004746329 92.30769 <NA>
## 108 OTU_45 0.004653096 100.00000 Dinophyceae
## 2 OTU_10 0.004589987 91.20879 <NA>
## 71 OTU_19 0.004441561 100.00000 <NA>
## 107 OTU_44 0.004392746 47.25275 other_Prasinophyceae
## 103 OTU_40 0.004178793 100.00000 Dinophyceae
## 61 OTU_17 0.004115244 93.40659 <NA>
## 85 OTU_28 0.004074785 96.70330 <NA>
## 11 OTU_106 0.003947248 86.81319 <NA>
## 114 OTU_50 0.003945049 96.70330 <NA>
## 53 OTU_15 0.003870287 53.84615 <NA>
## 130 OTU_64 0.003798822 100.00000 <NA>
## 97 OTU_36 0.003714605 87.91209 <NA>
## 137 OTU_70 0.003661391 79.12088 <NA>
## 45 OTU_14 0.003650177 93.40659 <NA>
## 150 OTU_81 0.003527038 98.90110 <NA>
## 121 OTU_5618 0.003490317 95.60440 <NA>
## 119 OTU_55 0.003475584 100.00000 <NA>
## 88 OTU_30 0.003463050 72.52747 <NA>
## 89 OTU_31 0.003353325 94.50549 <NA>
## 99 OTU_38 0.003306928 92.30769 <NA>
## 115 OTU_51 0.003292196 87.91209 <NA>
## 100 OTU_39 0.003174115 98.90110 Dinophyceae
## 110 OTU_47 0.003157623 74.72527 <NA>
## 91 OTU_33 0.003107928 98.90110 Dinophyceae
## 139 OTU_72 0.003063070 98.90110 <NA>
## 153 OTU_84 0.002979512 75.82418 <NA>
## 131 OTU_65 0.002933335 98.90110 <NA>
## 96 OTU_35799 0.002930696 100.00000 Dinophyceae
## 123 OTU_58 0.002866928 73.62637 <NA>
## 112 OTU_49 0.002866268 98.90110 Dinophyceae
## 104 OTU_41 0.002826688 95.60440 <NA>
## 116 OTU_52 0.002813715 86.81319 <NA>
## 26 OTU_119 0.002804039 28.57143 <NA>
## 105 OTU_42 0.002776773 94.50549 <NA>
## 98 OTU_37 0.002775674 95.60440 <NA>
## 109 OTU_46 0.002696513 27.47253 other_Prasinophyceae
## 106 OTU_43 0.002641541 69.23077 <NA>
## 142 OTU_75 0.002623729 97.80220 <NA>
## 111 OTU_48 0.002619332 92.30769 <NA>
## 138 OTU_71 0.002608337 100.00000 <NA>
## 132 OTU_66 0.002552925 97.80220 Dinophyceae
## 135 OTU_69 0.002535114 57.14286 <NA>
## 94 OTU_35 0.002530716 87.91209 <NA>
## 86 OTU_29 0.002500811 98.90110 <NA>
## 134 OTU_68 0.002456613 96.70330 <NA>
## 141 OTU_74 0.002430446 93.40659 <NA>
## 4 OTU_101 0.002352385 84.61538 <NA>
## 120 OTU_56 0.002344689 60.43956 <NA>
## 149 OTU_80 0.002281800 69.23077 <NA>
## 147 OTU_7889 0.002262010 83.51648 <NA>
## 129 OTU_6315 0.002235843 91.20879 <NA>
## 127 OTU_62 0.002210556 83.51648 <NA>
## 44 OTU_137 0.002194944 64.83516 <NA>
## 146 OTU_78 0.002190546 98.90110 Dinophyceae
## 151 OTU_82 0.002164819 90.10989 <NA>
## 154 OTU_85 0.002146568 91.20879 <NA>
## 140 OTU_73 0.002120621 72.52747 <NA>
## 90 OTU_32 0.002119521 75.82418 <NA>
## 166 OTU_96 0.002094674 67.03297 <NA>
## 157 OTU_89 0.002070926 73.62637 <NA>
## 117 OTU_53 0.002056193 41.75824 <NA>
## 56 OTU_16 0.002050476 35.16484 Prasinophyceae_clade-VII
## 162 OTU_92 0.002016393 75.82418 <NA>
## 155 OTU_87 0.001954384 92.30769 Dictyochophyceae
## 118 OTU_54 0.001933494 94.50549 <NA>
## 5 OTU_102 0.001932835 79.12088 <NA>
## 20 OTU_11454 0.001869946 100.00000 <NA>
## 143 OTU_76 0.001820031 91.20879 <NA>
## 22 OTU_116 0.001810796 82.41758 <NA>
## 13 OTU_109 0.001771435 68.13187 <NA>
## 133 OTU_67 0.001744609 97.80220 Dinophyceae
## 160 OTU_90 0.001727897 98.90110 <NA>
## 124 OTU_59 0.001708327 79.12088 <NA>
## 34 OTU_126 0.001694913 97.80220 <NA>
## 29 OTU_121 0.001675783 98.90110 Dinophyceae
## 126 OTU_61 0.001672045 82.41758 <NA>
## 32 OTU_124 0.001663909 85.71429 <NA>
## 15 OTU_110 0.001660171 84.61538 Dinophyceae
## 122 OTU_57 0.001648956 93.40659 <NA>
## 167 OTU_98 0.001593764 84.61538 <NA>
## 152 OTU_83 0.001576832 68.13187 <NA>
## 165 OTU_95 0.001555943 85.71429 <NA>
## 9 OTU_104 0.001548686 82.41758 <NA>
## 12 OTU_108 0.001526697 73.62637 <NA>
## 8 OTU_103 0.001507347 91.20879 <NA>
## 145 OTU_77 0.001438741 68.13187 Dinophyceae
## 101 OTU_3988 0.001435443 80.21978 <NA>
## 10 OTU_105 0.001416532 47.25275 <NA>
## 16 OTU_111 0.001409056 94.50549 Dinophyceae
## 70 OTU_1882 0.001406857 69.23077 <NA>
## 156 OTU_88 0.001394543 92.30769 <NA>
## 17 OTU_112 0.001392784 94.50549 Dinophyceae
## 42 OTU_135 0.001376293 89.01099 Chrysophyceae
## 69 OTU_188 0.001350785 96.70330 Dinophyceae
## 164 OTU_94 0.001324618 94.50549 <NA>
## 19 OTU_1136 0.001311865 98.90110 Dinophyceae
## 81 OTU_243 0.001305268 96.70330 <NA>
## 128 OTU_63 0.001297352 52.74725 Prasinophyceae_clade-VII
## 21 OTU_115 0.001292294 46.15385 <NA>
## 168 OTU_99 0.001284818 68.13187 <NA>
## 39 OTU_131 0.001274044 96.70330 Dictyochophyceae
## 58 OTU_162 0.001267007 76.92308 <NA>
## 50 OTU_145 0.001257332 89.01099 <NA>
## 63 OTU_177 0.001242379 92.30769 <NA>
## 66 OTU_180 0.001229626 68.13187 <NA>
## 33 OTU_125 0.001224348 96.70330 <NA>
## 163 OTU_93 0.001219291 89.01099 <NA>
## 48 OTU_142 0.001211815 73.62637 Prasinophyceae_clade-IX
## 30 OTU_122 0.001210275 67.03297 <NA>
## 51 OTU_146 0.001206757 68.13187 <NA>
## 95 OTU_35494 0.001198841 67.03297 <NA>
## 40 OTU_132 0.001194443 59.34066 <NA>
## 72 OTU_192 0.001191365 95.60440 <NA>
## 67 OTU_1819 0.001182789 71.42857 <NA>
## 79 OTU_235 0.001176192 83.51648 <NA>
## 57 OTU_161 0.001167177 51.64835 <NA>
## 35 OTU_128 0.001149586 85.71429 <NA>
## 25 OTU_11833 0.001148266 83.51648 <NA>
## 60 OTU_165 0.001145408 80.21978 <NA>
## 161 OTU_91 0.001142769 67.03297 <NA>
## 43 OTU_136 0.001140350 51.64835 <NA>
## 46 OTU_140 0.001132874 81.31868 <NA>
## 64 OTU_179 0.001126058 74.72527 <NA>
## 62 OTU_174 0.001119901 72.52747 <NA>
## 6 OTU_10246 0.001114623 74.72527 <NA>
## 18 OTU_113 0.001114183 74.72527 <NA>
## 144 OTU_767 0.001111545 32.96703 <NA>
## 47 OTU_141 0.001104069 65.93407 <NA>
## 36 OTU_129 0.001093954 64.83516 Chrysophyceae
## 38 OTU_130 0.001092634 100.00000 <NA>
## 92 OTU_338 0.001066247 96.70330 Dinophyceae
## 28 OTU_120 0.001066247 69.23077 <NA>
## 158 OTU_8924 0.001059211 72.52747 <NA>
## 41 OTU_133 0.001054153 57.14286 <NA>
## 68 OTU_1842 0.001048436 95.60440 Dinophyceae
## 54 OTU_151 0.001046237 67.03297 Chrysophyceae
## 23 OTU_117 0.001045798 92.30769 <NA>
## 31 OTU_123 0.001043379 74.72527 <NA>
## 59 OTU_163 0.001037662 76.92308 <NA>
## 77 OTU_220 0.001021610 94.50549 <NA>
## 7 OTU_10256 0.001017432 93.40659 Dinophyceae
## 49 OTU_144 0.001016552 91.20879 <NA>
## 55 OTU_157 0.001015013 41.75824 <NA>
## 3 OTU_100 0.001012374 68.13187 <NA>
## 24 OTU_118 0.001010175 69.23077 <NA>
## SILVA_classif
## 125 EF172947.1.1553_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 73 FJ832119.1.1585_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_marine_picoplankton
## 78 FR874416.1.1792_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_marine_picoeukaryote
## 148 EU780613.1.1717_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_eukaryote
## 82 AY664971.1.1755_Eukaryota;SAR;Alveolata;SCM37C52;uncultured_eukaryote
## 84 KC488491.1.1699_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_III;uncultured_marine_alveolate
## 1 KC488417.1.1688_Eukaryota;SAR;Alveolata;Dinoflagellata;Dinophyceae;Gymnodiniphycidae;Gymnodinium_clade;FV18-2D9;uncultured_dinoflagellate
## 27 EU793930.1.1664_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_syndiniales
## 136 KC582919.1.1797_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_alveolate
## 159 EF620525.1.1716_Eukaryota;SAR;Stramenopiles;Bicosoecida;Cafeteria;uncultured_bicosoecid
## 74 EF455763.1.1872_Eukaryota;SAR;Stramenopiles;Ochrophyta;Pelagophyceae;Pelagomonadales;Pelagomonas;Pelagomonas_calceolata
## 102 KC488405.1.1558_Eukaryota;SAR;Alveolata;Dinoflagellata;Dinophyceae;Gymnodiniphycidae;Gymnodinium_clade;Erythropsidinium;uncultured_dinoflagellate
## 37 JX188350.1.1750_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 14 JX188308.1.1760_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_III;uncultured_eukaryote
## 52 FJ832119.1.1585_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_marine_picoplankton
## 65 AY664924.1.1754_Eukaryota;SAR;Alveolata;OLI11255;uncultured_eukaryote
## 80 EU780594.1.1712_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_eukaryote
## 87 EF172960.1.1674_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_eukaryote
## 93 JQ782092.1.1744_Eukaryota;SAR;Stramenopiles;Ochrophyta;Chrysophyceae;uncultured;uncultured_stramenopile
## 75 KC488485.1.1680_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_marine_alveolate
## 83 EF172986.1.1644_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 113 EU793901.1.1663_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_syndiniales
## 76 KC582930.1.1796_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_alveolate
## 108 GU825491.1.1212_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Amoebophrya;uncultured_eukaryote
## 2 EU793418.1.1665_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_syndiniales
## 71 JX188306.1.1756_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_V;uncultured_eukaryote
## 107 KC583113.1.1768_Eukaryota;Archaeplastida;Chloroplastida;Chlorophyta;Mamiellophyceae;Bathycoccus;uncultured_Chlorophyta
## 103 KC582936.1.1795_Eukaryota;SAR;Alveolata;Dinoflagellata;Dinophyceae;Gymnodiniphycidae;SCM27C9;uncultured_alveolate
## 61 KC582887.1.1798_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_alveolate
## 85 EU371330.1.1733_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_marine_eukaryote
## 11 AY665056.1.1740_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 114 JQ781967.1.1805_Eukaryota;SAR;Stramenopiles;MAST-4;uncultured_stramenopile
## 53 EU106851.1.1693_Eukaryota;SAR;Stramenopiles;Bicosoecida;Caecitellus;Caecitellus_sp._RCC1078
## 130 JX188299.1.1773_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 97 FJ832105.1.1591_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_marine_plankton
## 137 EU793901.1.1663_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_syndiniales
## 45 KC582897.1.1794_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_alveolate
## 150 JQ782026.1.1809_Eukaryota;SAR;Stramenopiles;MAST-3;MAST-3F;uncultured_stramenopile
## 121 AACY020216037.2.1465_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;marine_metagenome
## 119 GU823346.1.1201_Eukaryota;SAR;Stramenopiles;MAST-1;MAST-1D;uncultured_stramenopile
## 88 KC488492.1.1682_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_marine_alveolate
## 89 AY129057.1.1798_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_marine_eukaryote
## 99 KC488409.1.1674_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_dinoflagellate
## 115 AY665031.1.1749_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 100 FJ832106.1.1588_Eukaryota;SAR;Alveolata;Dinoflagellata;Dinophyceae;Gymnodiniphycidae;Gyrodinium;uncultured_marine_plankton
## 110 EF172947.1.1553_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 91 AY664962.1.1755_Eukaryota;SAR;Alveolata;Dinoflagellata;uncultured_eukaryote
## 139 EU371330.1.1733_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_marine_eukaryote
## 153 EU793371.1.1679_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_syndiniales
## 131 JF791018.1.1777_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_alveolate
## 96 AY664971.1.1755_Eukaryota;SAR;Alveolata;SCM37C52;uncultured_eukaryote
## 123 GU819935.1.1201_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Duboscquella;uncultured_dinoflagellate
## 112 KC488407.1.1693_Eukaryota;SAR;Alveolata;Dinoflagellata;Dinophyceae;B7;uncultured_dinoflagellate
## 104 EU793370.1.1678_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_V;uncultured_syndiniales
## 116 KC488460.1.1689_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_marine_alveolate
## 26 EU647057.1.1189_Eukaryota;Opisthokonta;Nucletmycea;Fungi;Dikarya;Basidiomycota;Pucciniomycotina;Microbotryomycetes;Sporidiobolales;uncultured_Pucciniomycotina
## 105 AY749516.1.1576_Eukaryota;Centrohelida;H26-1;uncultured_eukaryote
## 98 AY665036.1.1748_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 109 KC583129.1.1754_Eukaryota;Archaeplastida;Chloroplastida;Chlorophyta;Mamiellophyceae;uncultured_Chlorophyta
## 106 EU780623.1.1645_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 142 AJ402349.1.1758_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_III;eukaryote_clone_OLI11005
## 111 EU793381.1.1670_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_syndiniales
## 138 JQ782036.1.1785_Eukaryota;SAR;Stramenopiles;MAST-1;MAST-1C;uncultured_stramenopile
## 132 JX188327.1.1759_Eukaryota;SAR;Alveolata;Dinoflagellata;Dinophyceae;Gymnodiniphycidae;Kareniaceae;Karlodinium;uncultured_eukaryote
## 135 EF172922.1.1644_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_eukaryote
## 94 GU820026.1.1394_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_dinoflagellate
## 86 DQ222879.1.1791_Eukaryota;Picozoa;Picomonadea;uncultured_phototrophic_eukaryote
## 134 EF172986.1.1644_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 141 KC582890.1.1797_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_alveolate
## 4 FJ832116.1.1590_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_marine_plankton
## 120 EU793901.1.1663_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_syndiniales
## 149 KC488477.1.1670_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_marine_alveolate
## 147 JQ420086.1.1778_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_phytoplankton
## 129 EU793392.1.1594_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_V;uncultured_syndiniales
## 127 <NA>
## 44 EU561890.1.1731_Eukaryota;SAR;Stramenopiles;MAST-3;MAST-3A;uncultured_marine_eukaryote
## 146 KC582936.1.1795_Eukaryota;SAR;Alveolata;Dinoflagellata;Dinophyceae;Gymnodiniphycidae;SCM27C9;uncultured_alveolate
## 151 JX840917.1.1201_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_dinoflagellate
## 154 EU793367.1.1601_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_syndiniales
## 140 KC583009.1.1804_Eukaryota;SAR;Stramenopiles;MAST-4;uncultured_stramenopile
## 90 EU793925.1.1594_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_syndiniales
## 166 KC582949.1.1792_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_alveolate
## 157 KC583046.1.1798_Eukaryota;SAR;Rhizaria;Retaria;RAD_B;uncultured_Rhizaria
## 117 AY520446.1.1695_Eukaryota;SAR;Stramenopiles;Bicosoecida;Caecitellus;Caecitellus_parvulus
## 56 KF031720.1.1747_Eukaryota;Archaeplastida;Chloroplastida;Chlorophyta;Clade_VII;Subclade_B;uncultured_eukaryote
## 162 <NA>
## 155 <NA>
## 118 DQ145114.1.1728_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_alveolate
## 5 GU820026.1.1394_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_dinoflagellate
## 20 EU793918.1.1662_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_syndiniales
## 143 KC582884.1.1799_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_alveolate
## 22 KC582930.1.1796_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_alveolate
## 13 EF172904.1.1680_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_eukaryote
## 133 EU446398.1.1566_Eukaryota;SAR;Alveolata;Dinoflagellata;Dinophyceae;Gymnodiniphycidae;Kareniaceae;Karlodinium;uncultured_marine_eukaryote
## 160 AJ402349.1.1758_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_III;eukaryote_clone_OLI11005
## 124 GU246573.1.1787_Eukaryota;SAR;Rhizaria;Retaria;Acantharia;Arthracanthida;Acanthometra;Acanthometra_sp._2_7202
## 34 HQ868687.1.903_Eukaryota;Picozoa;Picomonadea;Picomonadida;uncultured_eukaryote
## 29 JX840913.1.1300_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_dinoflagellate
## 126 JN811213.1.1672_Eukaryota;SAR;Rhizaria;Retaria;Acantharia;Arthracanthida;Acanthometra;Xiphacantha_alata
## 32 KC582893.1.1795_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_alveolate
## 15 JX188356.1.1758_Eukaryota;SAR;Alveolata;SCM37C52;uncultured_eukaryote
## 122 FN263031.1.1789_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_alveolate
## 167 JX188283.1.1756_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_eukaryote
## 152 JN832733.1.1713_Eukaryota;Cryptophyceae;Kathablepharidae;Leucocryptos;uncultured_eukaryote
## 165 EF172986.1.1644_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 9 EU793688.1.1653_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_syndiniales
## 12 <NA>
## 8 <NA>
## 145 FJ626714.1.1769_Eukaryota;SAR;Alveolata;Dinoflagellata;Dinophyceae;SL163A10;uncultured_marine_eukaryote
## 101 GU820421.1.1200_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_V;uncultured_dinoflagellate
## 10 EF050072.1.1712_Eukaryota;SAR;Stramenopiles;Bicosoecida;Caecitellus;uncultured_picobiliphyte
## 16 AY664888.1.1754_Eukaryota;SAR;Alveolata;Dinoflagellata;Dinophyceae;SCM37C58;uncultured_eukaryote
## 70 JX188283.1.1756_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_eukaryote
## 156 EU793678.1.1564_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_syndiniales
## 17 EF526792.1.1343_Eukaryota;SAR;Alveolata;SCM37C52;uncultured_marine_eukaryote
## 42 KC582995.1.1780_Eukaryota;SAR;Stramenopiles;Ochrophyta;Chrysophyceae;E222;uncultured_stramenopile
## 69 EU780618.1.1706_Eukaryota;SAR;Alveolata;Dinoflagellata;Dinophyceae;Gymnodiniphycidae;SCM27C9;uncultured_eukaryote
## 164 EU780603.1.1587_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 19 JX188293.1.1759_Eukaryota;SAR;Alveolata;SCM37C52;uncultured_eukaryote
## 81 JX840907.1.1356_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Amoebophrya;uncultured_dinoflagellate
## 128 KC583085.1.1776_Eukaryota;Archaeplastida;Chloroplastida;Chlorophyta;Clade_VII;Subclade_A;uncultured_Chlorophyta
## 21 <NA>
## 168 KC488471.1.1700_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_marine_alveolate
## 39 KC582988.1.1798_Eukaryota;SAR;Stramenopiles;Ochrophyta;Dictyochophyceae;Pedinellales;uncultured_stramenopile
## 58 <NA>
## 50 KC582941.1.1793_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_alveolate
## 63 EU780589.1.1635_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 66 KC582949.1.1792_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_alveolate
## 33 GU823346.1.1201_Eukaryota;SAR;Stramenopiles;MAST-1;MAST-1D;uncultured_stramenopile
## 163 EU793840.1.1684_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_syndiniales
## 48 JX188280.1.1747_Eukaryota;Archaeplastida;Chloroplastida;Chlorophyta;Clade_IX;uncultured_eukaryote
## 30 <NA>
## 51 <NA>
## 95 KF031708.1.1743_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 40 <NA>
## 72 GU819857.1.1220_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_dinoflagellate
## 67 KC582919.1.1797_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_alveolate
## 79 EF172986.1.1644_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 57 JX840914.1.1343_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_dinoflagellate
## 35 KC582920.1.1797_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_alveolate
## 25 EU793930.1.1664_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_syndiniales
## 60 JQ782054.1.1813_Eukaryota;SAR;Stramenopiles;MAST-4;uncultured_stramenopile
## 161 EF172944.1.1676_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 43 KC582949.1.1792_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_alveolate
## 46 AY665036.1.1748_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 64 AY665056.1.1740_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 62 JX840900.1.1305_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_dinoflagellate
## 6 AY129066.1.1807_Eukaryota;SAR;Stramenopiles;MAST-4;MAST-4B;uncultured_marine_eukaryote
## 18 GU820026.1.1394_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_dinoflagellate
## 144 JF826336.1.1604_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_marine_alveolate
## 47 JX188350.1.1750_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 36 FJ537356.1.1779_Eukaryota;SAR;Stramenopiles;Ochrophyta;Chrysophyceae;LG01-09;uncultured_marine_chrysophyte
## 38 JX173254.1.1528_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Amoebophrya;Amoebophrya_sp._ex_Alexandrium_sp
## 92 AF022199.1.1803_Eukaryota;SAR;Alveolata;Dinoflagellata;Dinophyceae;Gymnodiniphycidae;Gymnodinium_clade;Lepidodinium;Lepidodinium_viride
## 28 <NA>
## 158 FJ914455.1.1433_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_marine_dinoflagellate
## 41 JN832831.1.1719_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_eukaryote
## 68 JX840913.1.1300_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_dinoflagellate
## 54 FJ537347.1.1751_Eukaryota;SAR;Stramenopiles;Ochrophyta;Chrysophyceae;E222;uncultured_marine_chrysophyte
## 23 GQ365865.1.1537_Eukaryota;Incertae_Sedis;Telonema;uncultured_eukaryote
## 31 KC488339.1.1675_Eukaryota;Picozoa;Picomonadea;Picomonadida;uncultured_biliphyte
## 59 KC582881.1.1799_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_alveolate
## 77 AF239260.1.1699_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Amoebophrya;Amoebophrya_sp._'Dinophysis'
## 7 KC488407.1.1693_Eukaryota;SAR;Alveolata;Dinoflagellata;Dinophyceae;B7;uncultured_dinoflagellate
## 49 JN832733.1.1713_Eukaryota;Cryptophyceae;Kathablepharidae;Leucocryptos;uncultured_eukaryote
## 55 EF173015.1.1676_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 3 DQ186530.1.1762_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_alveolate
## 24 GU823500.1.1392_Eukaryota;SAR;Stramenopiles;MAST-7;MAST-7B;uncultured_stramenopile
## MAS_classif
## 125 GQ913047_MALV-I_Alveolata
## 73 EU793773_MALV-I_Alveolata
## 78 EU793423_MALV-I_Alveolata
## 148 FJ537505_MALV-II_Alveolata
## 82 FJ537481_Dinophyceae_Alveolata
## 84 FJ431657_MALV-III_Alveolata
## 1 EU793855_Dinophyceae_Alveolata
## 27 EU793468_MALV-II_Alveolata
## 136 EU793854_MALV-II_Alveolata
## 159 AF174365_Bicosoecids_Stramenopiles
## 74 U14389_Pelagophytes_Stramenopiles
## 102 EU780636_Dinophyceae_Alveolata
## 37 EU793270_MALV-I_Alveolata
## 14 GQ382836_MALV-III_Alveolata
## 52 AY426873_MALV-I_Alveolata
## 65 EU793623_Dinophyceae_Alveolata
## 80 EU793869_MALV-II_Alveolata
## 87 EU793316_MALV-II_Alveolata
## 93 EF633325_Chrysophytes_Stramenopiles
## 75 EU561962_MALV-I_Alveolata
## 83 EU793161_MALV-I_Alveolata
## 113 EU818388_MALV-I_Alveolata
## 76 EU562164_MALV-II_Alveolata
## 108 EU793487_Dinophyceae_Alveolata
## 2 EU818180_MALV-II_Alveolata
## 71 EU793472_MALV-V_Alveolata
## 107 ZZ0025910_Prasinophyceae_Chlorophyta
## 103 EU793835_Dinophyceae_Alveolata
## 61 EU793396_MALV-II_Alveolata
## 85 EU793876_MALV-I_Alveolata
## 11 EU818437_MALV-I_Alveolata
## 114 JQ782043_MAST-4A_Stramenopiles
## 53 EU106844_Bicosoecids_Stramenopiles
## 130 EU793397_MALV-I_Alveolata
## 97 EU793662_MALV-II_Alveolata
## 137 EU817894_MALV-I_Alveolata
## 45 EU793496_MALV-II_Alveolata
## 150 EF172961_MAST-3A_Stramenopiles
## 121 EU793591_MALV-II_Alveolata
## 119 JQ781940_MAST-1D_Stramenopiles
## 88 EU793526_MALV-II_Alveolata
## 89 AY129057_MALV-II_Alveolata
## 99 EU818099_MALV-I_Alveolata
## 115 EU793570_MALV-I_Alveolata
## 100 EU561942_Dinophyceae_Alveolata
## 110 EU793333_MALV-I_Alveolata
## 91 GQ382841_Dinophyceae_Alveolata
## 139 EU793665_MALV-I_Alveolata
## 153 EU793307_MALV-I_Alveolata
## 131 EU793454_MALV-II_Alveolata
## 96 FJ537481_Dinophyceae_Alveolata
## 123 EU562061_MALV-I_Alveolata
## 112 AY426916_Dinophyceae_Alveolata
## 104 EU562105_MALV-V_Alveolata
## 116 AY665057_MALV-I_Alveolata
## 26 AB021668_Basidiomycota_Fungi
## 105 EU500119_Centroheliozoa_Hacrobia
## 98 EU793849_MALV-I_Alveolata
## 109 EU561670_Prasinophyceae_Chlorophyta
## 106 EU780623_MALV-I_Alveolata
## 142 DQ917937_MALV-III_Alveolata
## 111 EU793900_MALV-II_Alveolata
## 138 JQ782036_MAST-1C_Stramenopiles
## 132 EU793310_Dinophyceae_Alveolata
## 135 EU818483_MALV-II_Alveolata
## 94 FJ537453_MALV-II_Alveolata
## 86 ZZ0026100_Picobiliphyta_Hacrobia
## 134 EU817904_MALV-I_Alveolata
## 141 EU793160_MALV-II_Alveolata
## 4 EU793860_MALV-II_Alveolata
## 120 EU817899_MALV-I_Alveolata
## 149 EU818332_MALV-II_Alveolata
## 147 EU561991_MALV-I_Alveolata
## 129 EU793880_MALV-V_Alveolata
## 127 EU818263_MALV-II_Alveolata
## 44 GQ863794_MAST-3A_Stramenopiles
## 146 EU818104_Dinophyceae_Alveolata
## 151 FJ537409_MALV-II_Alveolata
## 154 EU793151_MALV-II_Alveolata
## 140 EF172962_MAST-4C_Stramenopiles
## 90 EU793455_MALV-II_Alveolata
## 166 EU793858_MALV-I_Alveolata
## 157 <NA>
## 117 EF620527_Bicosoecids_Stramenopiles
## 56 FJ537299_Prasinophyceae_Chlorophyta
## 162 EU793851_MALV-I_Alveolata
## 155 <NA>
## 118 EU793359_MALV-I_Alveolata
## 5 DQ918857_MALV-II_Alveolata
## 20 GQ913054_MALV-I_Alveolata
## 143 EU817983_MALV-II_Alveolata
## 22 AY937485_MALV-II_Alveolata
## 13 FJ431848_MALV-II_Alveolata
## 133 EU500204_Dinophyceae_Alveolata
## 160 EU793515_MALV-III_Alveolata
## 124 AJ402332_Acantharea_Rhizaria
## 34 EF539143_Picobiliphyta_Hacrobia
## 29 EU561774_Dinophyceae_Alveolata
## 126 GQ913026_Acantharea_Rhizaria
## 32 EU793353_MALV-II_Alveolata
## 15 EU561885_Dinophyceae_Alveolata
## 122 GQ382775_MALV-I_Alveolata
## 167 EU817986_MALV-II_Alveolata
## 152 EU561799_Katablepharidales_Hacrobia
## 165 EU793360_MALV-I_Alveolata
## 9 EU793411_MALV-II_Alveolata
## 12 EU561778_MALV-II_Alveolata
## 8 EU500081_MAST-25_Stramenopiles
## 145 AY938395_Dinophyceae_Alveolata
## 101 EU793284_MALV-V_Alveolata
## 10 AY827847_Bicosoecids_Stramenopiles
## 16 EU780632_Dinophyceae_Alveolata
## 70 EU817986_MALV-II_Alveolata
## 156 EU818216_MALV-II_Alveolata
## 17 EF526792_Dinophyceae_Alveolata
## 42 EF172998_Chrysophytes_Stramenopiles
## 69 EU780618_Dinophyceae_Alveolata
## 164 EU561765_MALV-I_Alveolata
## 19 EU793725_Dinophyceae_Alveolata
## 81 AJ965134_MALV-II_Alveolata
## 128 AY425303_Prasinophyceae_Chlorophyta
## 21 EU562016_MALV-I_Alveolata
## 168 EU793469_MALV-II_Alveolata
## 39 AB241.O08_Dictyochophytes_Stramenopiles
## 58 ZZ0071148_MALV-II_Alveolata
## 50 FJ537539_MALV-II_Alveolata
## 63 DQ918674_MALV-I_Alveolata
## 66 EU793313_MALV-I_Alveolata
## 33 EF173010_MAST-1D_Stramenopiles
## 163 EU793592_MALV-II_Alveolata
## 48 FJ537325_Prasinophyceae_Chlorophyta
## 30 EU500010_MALV-I_Alveolata
## 51 EU818469_MALV-II_Alveolata
## 95 DQ918838_MALV-I_Alveolata
## 40 <NA>
## 72 GQ382521_MALV-I_Alveolata
## 67 AY295383_MALV-II_Alveolata
## 79 EU793161_MALV-I_Alveolata
## 57 FJ431832_MALV-II_Alveolata
## 35 EU793410_MALV-II_Alveolata
## 25 EU793468_MALV-II_Alveolata
## 60 HQ869203_MAST-4E_Stramenopiles
## 161 EU561794_MALV-I_Alveolata
## 43 EU793509_MALV-I_Alveolata
## 46 EU793607_MALV-I_Alveolata
## 64 EU817989_MALV-I_Alveolata
## 62 EU793203_MALV-II_Alveolata
## 6 AY129066_MAST-4B_Stramenopiles
## 18 EU793134_MALV-II_Alveolata
## 144 EU793879_MALV-I_Alveolata
## 47 EU793500_MALV-I_Alveolata
## 36 FJ537351_Chrysophytes_Stramenopiles
## 38 EU793906_MALV-II_Alveolata
## 92 AY938154_Dinophyceae_Alveolata
## 28 GQ382358_MALV-I_Alveolata
## 158 EU793914_MALV-II_Alveolata
## 41 GQ913086_MALV-II_Alveolata
## 68 EU793623_Dinophyceae_Alveolata
## 54 AY129063_Chrysophytes_Stramenopiles
## 23 DQ119999_Telonemia_Hacrobia
## 31 EF172850_Picobiliphyta_Hacrobia
## 59 EF172987_MALV-II_Alveolata
## 77 GQ383169_MALV-II_Alveolata
## 7 AY426916_Dinophyceae_Alveolata
## 49 EU561799_Katablepharidales_Hacrobia
## 55 EU818510_MALV-I_Alveolata
## 3 FJ431830_MALV-II_Alveolata
## 24 EU499985_MAST-7B_Stramenopiles
## BM_classif
## 125 32_MALV-I_Alveolata_15673
## 73 3261_MALV-I_Alveolata_385
## 78 75_MALV-I_Alveolata_3817
## 148 173_MALV-II_Alveolata_516
## 82 5651_Dinophyceae_Alveolata_249
## 84 38_MALV-III_Alveolata_6570
## 1 9492_Dinophyceae_Alveolata_15
## 27 159_MALV-II_Alveolata_1129
## 136 549_MALV-II_Alveolata_410
## 159 <NA>
## 74 30_Pelagophyceae_Stramenopiles_3903
## 102 21474_Dinophyceae_Alveolata_6
## 37 110_MALV-I_Alveolata_871
## 14 70_MALV-III_Alveolata_4778
## 52 3261_MALV-I_Alveolata_385
## 65 16659_Dinophyceae_Alveolata_16
## 80 2230_MALV-II_Alveolata_177
## 87 <NA>
## 93 2777_Chrysophyceae_Stramenopiles_14
## 75 408_MALV-I_Alveolata_437
## 83 12073_MALV-I_Alveolata_80
## 113 398_MALV-I_Alveolata_395
## 76 437_MALV-II_Alveolata_11
## 108 19832_Dinophyceae_Alveolata_6
## 2 437_MALV-II_Alveolata_11
## 71 269_MALV-V_Alveolata_430
## 107 31_Mamiellophyceae_Archaeplastida_4744
## 103 1849_Dinophyceae_Alveolata_250
## 61 538_MALV-II_Alveolata_49
## 85 90_MALV-I_Alveolata_1447
## 11 90_MALV-I_Alveolata_1447
## 114 127_MAST-4_Stramenopiles_1171
## 53 <NA>
## 130 10705_MALV-I_Alveolata_87
## 97 234_MALV-II_Alveolata_626
## 137 398_MALV-I_Alveolata_395
## 45 396_MALV-II_Alveolata_141
## 150 5352_MAST-3_Stramenopiles_16
## 121 159_MALV-II_Alveolata_1129
## 119 135_MAST-1_Stramenopiles_1096
## 88 437_MALV-II_Alveolata_11
## 89 372_MALV-II_Alveolata_174
## 99 161_MALV-I_Alveolata_1735
## 115 195_MALV-I_Alveolata_265
## 100 60_Dinophyceae_Alveolata_2847
## 110 32_MALV-I_Alveolata_15673
## 91 5854_Dinophyceae_Alveolata_118
## 139 2689_MALV-I_Alveolata_92
## 153 83_MALV-I_Alveolata_2111
## 131 11869_MALV-II_Alveolata_32
## 96 5651_Dinophyceae_Alveolata_249
## 123 6219_MALV-I_Alveolata_13
## 112 63_Dinophyceae_Alveolata_4010
## 104 269_MALV-V_Alveolata_430
## 116 152_MALV-I_Alveolata_1517
## 26 3036_Basidiomycota_Fungi_13
## 105 228_Centrohelida_Hacrobia_542
## 98 320_MALV-I_Alveolata_280
## 109 13549_Mamiellophyceae_Archaeplastida_22
## 106 140_MALV-I_Alveolata_296
## 142 92_MALV-III_Alveolata_2457
## 111 666_MALV-II_Alveolata_7
## 138 129_MAST-1_Stramenopiles_1402
## 132 250_Dinophyceae_Alveolata_874
## 135 592_MALV-II_Alveolata_99
## 94 779_MALV-II_Alveolata_39
## 86 96_Picozoa_Hacrobia_1708
## 134 10506_MALV-I_Alveolata_3
## 141 6845_MALV-II_Alveolata_162
## 4 12035_MALV-II_Alveolata_25
## 120 398_MALV-I_Alveolata_395
## 149 185_MALV-II_Alveolata_315
## 147 75_MALV-I_Alveolata_3817
## 129 269_MALV-V_Alveolata_430
## 127 698_MALV-II_Alveolata_96
## 44 1130_MAST-3_Stramenopiles_92
## 146 181_Dinophyceae_Alveolata_934
## 151 666_MALV-II_Alveolata_7
## 154 143_MALV-II_Alveolata_365
## 140 891_MAST-4_Stramenopiles_115
## 90 1440_MALV-II_Alveolata_413
## 166 168_MALV-I_Alveolata_655
## 157 863_Polycystinea_Rhizaria_40
## 117 2638_Bicosoecida_Stramenopiles_17
## 56 1062_Prasinophyceae_Archaeplastida_88
## 162 <NA>
## 155 512_Dictyochophyceae_Stramenopiles_107
## 118 110_MALV-I_Alveolata_871
## 5 583_MALV-II_Alveolata_48
## 20 10705_MALV-I_Alveolata_87
## 143 1768_MALV-II_Alveolata_24
## 22 437_MALV-II_Alveolata_11
## 13 592_MALV-II_Alveolata_99
## 133 9883_Dinophyceae_Alveolata_11
## 160 373_MALV-III_Alveolata_220
## 124 1_Acantharia_Rhizaria_54126
## 34 64_Picozoa_Hacrobia_4926
## 29 2659_Dinophyceae_Alveolata_438
## 126 46_Acantharia_Rhizaria_1903
## 32 1019_MALV-II_Alveolata_76
## 15 3063_Dinophyceae_Alveolata_28
## 122 5250_MALV-I_Alveolata_344
## 167 173_MALV-II_Alveolata_516
## 152 299_Katablepharidae_Hacrobia_783
## 165 9794_MALV-I_Alveolata_9
## 9 183_MALV-II_Alveolata_589
## 12 <NA>
## 8 470_MAST-25_Stramenopiles_101
## 145 19112_Dinophyceae_Alveolata_32
## 101 269_MALV-V_Alveolata_430
## 10 2638_Bicosoecida_Stramenopiles_17
## 16 15157_Dinophyceae_Alveolata_44
## 70 9452_MALV-II_Alveolata_18
## 156 183_MALV-II_Alveolata_589
## 17 4619_Dinophyceae_Alveolata_56
## 42 524_Chrysophyceae_Stramenopiles_220
## 69 6794_Dinophyceae_Alveolata_12
## 164 274_MALV-I_Alveolata_291
## 19 12262_Dinophyceae_Alveolata_20
## 81 449_MALV-II_Alveolata_595
## 128 91_Prasinophyceae_Archaeplastida_63
## 21 812_MALV-I_Alveolata_38
## 168 9413_MALV-II_Alveolata_19
## 39 1696_Dictyochophyceae_Stramenopiles_41
## 58 <NA>
## 50 15297_MALV-II_Alveolata_17
## 63 979_MALV-I_Alveolata_83
## 66 168_MALV-I_Alveolata_655
## 33 5093_MAST-1_Stramenopiles_47
## 163 13359_MALV-II_Alveolata_15
## 48 724_Prasinophyceae_Archaeplastida_125
## 30 <NA>
## 51 1531_MALV-II_Alveolata_17
## 95 152_MALV-I_Alveolata_1517
## 40 1257_Acantharia_Rhizaria_46
## 72 4958_MALV-I_Alveolata_259
## 67 549_MALV-II_Alveolata_410
## 79 12073_MALV-I_Alveolata_80
## 57 3663_MALV-II_Alveolata_17
## 35 1019_MALV-II_Alveolata_76
## 25 159_MALV-II_Alveolata_1129
## 60 256_MAST-4_Stramenopiles_625
## 161 879_MALV-I_Alveolata_129
## 43 168_MALV-I_Alveolata_655
## 46 320_MALV-I_Alveolata_280
## 64 <NA>
## 62 8096_MALV-II_Alveolata_7
## 6 127_MAST-4_Stramenopiles_1171
## 18 1073_MALV-II_Alveolata_82
## 144 3261_MALV-I_Alveolata_385
## 47 110_MALV-I_Alveolata_871
## 36 218_Chrysophyceae_Stramenopiles_618
## 38 329_MALV-II_Alveolata_831
## 92 9_Dinophyceae_Alveolata_61014
## 28 <NA>
## 158 9413_MALV-II_Alveolata_19
## 41 1073_MALV-II_Alveolata_82
## 68 13136_Dinophyceae_Alveolata_79
## 54 556_Chrysophyceae_Stramenopiles_160
## 23 201_Telonema_Hacrobia_877
## 31 18617_Picozoa_Hacrobia_21
## 59 815_MALV-II_Alveolata_98
## 77 431_MALV-II_Alveolata_283
## 7 63_Dinophyceae_Alveolata_4010
## 49 299_Katablepharidae_Hacrobia_783
## 55 13516_MALV-I_Alveolata_25
## 3 234_MALV-II_Alveolata_626
## 24 87_MAST-7_Stramenopiles_1839Number and roportion of regionally abundant OTUs (%):
## [1] 168## [1] 0.4382075
Cosmopolitan OTUs (relative abundance over 0.1% and occurence in more than 80% of samples):
## otu_names mean_rabund perc_occur SILVA_plus_MAS_plus_BM_classif
## 79 OTU_6 0.037234661 100.00000 <NA>
## 40 OTU_2 0.035301387 100.00000 <NA>
## 48 OTU_25 0.010648841 100.00000 Dinophyceae
## 50 OTU_27 0.010030730 100.00000 <NA>
## 1 OTU_1 0.008782193 100.00000 Dinophyceae
## 17 OTU_12 0.008684342 100.00000 <NA>
## 88 OTU_7 0.007983332 100.00000 <NA>
## 64 OTU_4 0.007484402 100.00000 Dinophyceae
## 35 OTU_18 0.006813077 100.00000 Dinophyceae
## 68 OTU_45 0.004653096 100.00000 Dinophyceae
## 38 OTU_19 0.004441561 100.00000 <NA>
## 65 OTU_40 0.004178793 100.00000 Dinophyceae
## 83 OTU_64 0.003798822 100.00000 <NA>
## 76 OTU_55 0.003475584 100.00000 <NA>
## 58 OTU_35799 0.002930696 100.00000 Dinophyceae
## 89 OTU_71 0.002608337 100.00000 <NA>
## 13 OTU_11454 0.001869946 100.00000 <NA>
## 24 OTU_130 0.001092634 100.00000 <NA>
## 96 OTU_8 0.011335998 98.90110 <NA>
## 23 OTU_13 0.007211298 98.90110 <NA>
## 8 OTU_11 0.007044621 98.90110 <NA>
## 46 OTU_24 0.006641343 98.90110 <NA>
## 97 OTU_81 0.003527038 98.90110 <NA>
## 62 OTU_39 0.003174115 98.90110 Dinophyceae
## 55 OTU_33 0.003107928 98.90110 Dinophyceae
## 90 OTU_72 0.003063070 98.90110 <NA>
## 84 OTU_65 0.002933335 98.90110 <NA>
## 70 OTU_49 0.002866268 98.90110 Dinophyceae
## 52 OTU_29 0.002500811 98.90110 <NA>
## 94 OTU_78 0.002190546 98.90110 Dinophyceae
## 102 OTU_90 0.001727897 98.90110 <NA>
## 18 OTU_121 0.001675783 98.90110 Dinophyceae
## 12 OTU_1136 0.001311865 98.90110 Dinophyceae
## 92 OTU_75 0.002623729 97.80220 <NA>
## 85 OTU_66 0.002552925 97.80220 Dinophyceae
## 86 OTU_67 0.001744609 97.80220 Dinophyceae
## 21 OTU_126 0.001694913 97.80220 <NA>
## 44 OTU_23 0.012482065 96.70330 <NA>
## 51 OTU_28 0.004074785 96.70330 <NA>
## 72 OTU_50 0.003945049 96.70330 <NA>
## 87 OTU_68 0.002456613 96.70330 <NA>
## 37 OTU_188 0.001350785 96.70330 Dinophyceae
## 47 OTU_243 0.001305268 96.70330 <NA>
## 25 OTU_131 0.001274044 96.70330 Dictyochophyceae
## 20 OTU_125 0.001224348 96.70330 <NA>
## 56 OTU_338 0.001066247 96.70330 Dinophyceae
## 31 OTU_14696 0.006932257 95.60440 <NA>
## 49 OTU_26 0.005116624 95.60440 <NA>
## 77 OTU_5618 0.003490317 95.60440 <NA>
## 66 OTU_41 0.002826688 95.60440 <NA>
## 60 OTU_37 0.002775674 95.60440 <NA>
## 39 OTU_192 0.001191365 95.60440 <NA>
## 36 OTU_1842 0.001048436 95.60440 Dinophyceae
## 41 OTU_21 0.005359603 94.50549 <NA>
## 54 OTU_31 0.003353325 94.50549 <NA>
## 67 OTU_42 0.002776773 94.50549 <NA>
## 75 OTU_54 0.001933494 94.50549 <NA>
## 10 OTU_111 0.001409056 94.50549 Dinophyceae
## 11 OTU_112 0.001392784 94.50549 Dinophyceae
## 104 OTU_94 0.001324618 94.50549 <NA>
## 43 OTU_220 0.001021610 94.50549 <NA>
## 33 OTU_17 0.004115244 93.40659 <NA>
## 27 OTU_14 0.003650177 93.40659 <NA>
## 91 OTU_74 0.002430446 93.40659 <NA>
## 78 OTU_57 0.001648956 93.40659 <NA>
## 4 OTU_10256 0.001017432 93.40659 Dinophyceae
## 42 OTU_22 0.004746329 92.30769 <NA>
## 61 OTU_38 0.003306928 92.30769 <NA>
## 69 OTU_48 0.002619332 92.30769 <NA>
## 100 OTU_87 0.001954384 92.30769 Dictyochophyceae
## 101 OTU_88 0.001394543 92.30769 <NA>
## 34 OTU_177 0.001242379 92.30769 <NA>
## 15 OTU_117 0.001045798 92.30769 <NA>
## 2 OTU_10 0.004589987 91.20879 <NA>
## 82 OTU_6315 0.002235843 91.20879 <NA>
## 99 OTU_85 0.002146568 91.20879 <NA>
## 93 OTU_76 0.001820031 91.20879 <NA>
## 5 OTU_103 0.001507347 91.20879 <NA>
## 29 OTU_144 0.001016552 91.20879 <NA>
## 98 OTU_82 0.002164819 90.10989 <NA>
## 26 OTU_135 0.001376293 89.01099 Chrysophyceae
## 30 OTU_145 0.001257332 89.01099 <NA>
## 103 OTU_93 0.001219291 89.01099 <NA>
## 59 OTU_36 0.003714605 87.91209 <NA>
## 73 OTU_51 0.003292196 87.91209 <NA>
## 57 OTU_35 0.002530716 87.91209 <NA>
## 7 OTU_106 0.003947248 86.81319 <NA>
## 74 OTU_52 0.002813715 86.81319 <NA>
## 53 OTU_3 0.005921862 85.71429 <NA>
## 19 OTU_124 0.001663909 85.71429 <NA>
## 105 OTU_95 0.001555943 85.71429 <NA>
## 22 OTU_128 0.001149586 85.71429 <NA>
## 3 OTU_101 0.002352385 84.61538 <NA>
## 9 OTU_110 0.001660171 84.61538 Dinophyceae
## 106 OTU_98 0.001593764 84.61538 <NA>
## 95 OTU_7889 0.002262010 83.51648 <NA>
## 81 OTU_62 0.002210556 83.51648 <NA>
## 45 OTU_235 0.001176192 83.51648 <NA>
## 16 OTU_11833 0.001148266 83.51648 <NA>
## 14 OTU_116 0.001810796 82.41758 <NA>
## 80 OTU_61 0.001672045 82.41758 <NA>
## 6 OTU_104 0.001548686 82.41758 <NA>
## 28 OTU_140 0.001132874 81.31868 <NA>
## 71 OTU_5 0.004815375 80.21978 <NA>
## 63 OTU_3988 0.001435443 80.21978 <NA>
## 32 OTU_165 0.001145408 80.21978 <NA>
## SILVA_classif
## 79 EF172947.1.1553_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 40 FJ832119.1.1585_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_marine_picoplankton
## 48 AY664971.1.1755_Eukaryota;SAR;Alveolata;SCM37C52;uncultured_eukaryote
## 50 KC488491.1.1699_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_III;uncultured_marine_alveolate
## 1 KC488417.1.1688_Eukaryota;SAR;Alveolata;Dinoflagellata;Dinophyceae;Gymnodiniphycidae;Gymnodinium_clade;FV18-2D9;uncultured_dinoflagellate
## 17 EU793930.1.1664_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_syndiniales
## 88 KC582919.1.1797_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_alveolate
## 64 KC488405.1.1558_Eukaryota;SAR;Alveolata;Dinoflagellata;Dinophyceae;Gymnodiniphycidae;Gymnodinium_clade;Erythropsidinium;uncultured_dinoflagellate
## 35 AY664924.1.1754_Eukaryota;SAR;Alveolata;OLI11255;uncultured_eukaryote
## 68 GU825491.1.1212_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Amoebophrya;uncultured_eukaryote
## 38 JX188306.1.1756_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_V;uncultured_eukaryote
## 65 KC582936.1.1795_Eukaryota;SAR;Alveolata;Dinoflagellata;Dinophyceae;Gymnodiniphycidae;SCM27C9;uncultured_alveolate
## 83 JX188299.1.1773_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 76 GU823346.1.1201_Eukaryota;SAR;Stramenopiles;MAST-1;MAST-1D;uncultured_stramenopile
## 58 AY664971.1.1755_Eukaryota;SAR;Alveolata;SCM37C52;uncultured_eukaryote
## 89 JQ782036.1.1785_Eukaryota;SAR;Stramenopiles;MAST-1;MAST-1C;uncultured_stramenopile
## 13 EU793918.1.1662_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_syndiniales
## 24 JX173254.1.1528_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Amoebophrya;Amoebophrya_sp._ex_Alexandrium_sp
## 96 EU780613.1.1717_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_eukaryote
## 23 JX188350.1.1750_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 8 JX188308.1.1760_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_III;uncultured_eukaryote
## 46 EU780594.1.1712_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_eukaryote
## 97 JQ782026.1.1809_Eukaryota;SAR;Stramenopiles;MAST-3;MAST-3F;uncultured_stramenopile
## 62 FJ832106.1.1588_Eukaryota;SAR;Alveolata;Dinoflagellata;Dinophyceae;Gymnodiniphycidae;Gyrodinium;uncultured_marine_plankton
## 55 AY664962.1.1755_Eukaryota;SAR;Alveolata;Dinoflagellata;uncultured_eukaryote
## 90 EU371330.1.1733_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_marine_eukaryote
## 84 JF791018.1.1777_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_alveolate
## 70 KC488407.1.1693_Eukaryota;SAR;Alveolata;Dinoflagellata;Dinophyceae;B7;uncultured_dinoflagellate
## 52 DQ222879.1.1791_Eukaryota;Picozoa;Picomonadea;uncultured_phototrophic_eukaryote
## 94 KC582936.1.1795_Eukaryota;SAR;Alveolata;Dinoflagellata;Dinophyceae;Gymnodiniphycidae;SCM27C9;uncultured_alveolate
## 102 AJ402349.1.1758_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_III;eukaryote_clone_OLI11005
## 18 JX840913.1.1300_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_dinoflagellate
## 12 JX188293.1.1759_Eukaryota;SAR;Alveolata;SCM37C52;uncultured_eukaryote
## 92 AJ402349.1.1758_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_III;eukaryote_clone_OLI11005
## 85 JX188327.1.1759_Eukaryota;SAR;Alveolata;Dinoflagellata;Dinophyceae;Gymnodiniphycidae;Kareniaceae;Karlodinium;uncultured_eukaryote
## 86 EU446398.1.1566_Eukaryota;SAR;Alveolata;Dinoflagellata;Dinophyceae;Gymnodiniphycidae;Kareniaceae;Karlodinium;uncultured_marine_eukaryote
## 21 HQ868687.1.903_Eukaryota;Picozoa;Picomonadea;Picomonadida;uncultured_eukaryote
## 44 FR874416.1.1792_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_marine_picoeukaryote
## 51 EU371330.1.1733_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_marine_eukaryote
## 72 JQ781967.1.1805_Eukaryota;SAR;Stramenopiles;MAST-4;uncultured_stramenopile
## 87 EF172986.1.1644_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 37 EU780618.1.1706_Eukaryota;SAR;Alveolata;Dinoflagellata;Dinophyceae;Gymnodiniphycidae;SCM27C9;uncultured_eukaryote
## 47 JX840907.1.1356_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Amoebophrya;uncultured_dinoflagellate
## 25 KC582988.1.1798_Eukaryota;SAR;Stramenopiles;Ochrophyta;Dictyochophyceae;Pedinellales;uncultured_stramenopile
## 20 GU823346.1.1201_Eukaryota;SAR;Stramenopiles;MAST-1;MAST-1D;uncultured_stramenopile
## 56 AF022199.1.1803_Eukaryota;SAR;Alveolata;Dinoflagellata;Dinophyceae;Gymnodiniphycidae;Gymnodinium_clade;Lepidodinium;Lepidodinium_viride
## 31 FJ832119.1.1585_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_marine_picoplankton
## 49 EF172986.1.1644_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 77 AACY020216037.2.1465_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;marine_metagenome
## 66 EU793370.1.1678_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_V;uncultured_syndiniales
## 60 AY665036.1.1748_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 39 GU819857.1.1220_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_dinoflagellate
## 36 JX840913.1.1300_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_dinoflagellate
## 41 KC488485.1.1680_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_marine_alveolate
## 54 AY129057.1.1798_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_marine_eukaryote
## 67 AY749516.1.1576_Eukaryota;Centrohelida;H26-1;uncultured_eukaryote
## 75 DQ145114.1.1728_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_alveolate
## 10 AY664888.1.1754_Eukaryota;SAR;Alveolata;Dinoflagellata;Dinophyceae;SCM37C58;uncultured_eukaryote
## 11 EF526792.1.1343_Eukaryota;SAR;Alveolata;SCM37C52;uncultured_marine_eukaryote
## 104 EU780603.1.1587_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 43 AF239260.1.1699_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Amoebophrya;Amoebophrya_sp._'Dinophysis'
## 33 KC582887.1.1798_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_alveolate
## 27 KC582897.1.1794_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_alveolate
## 91 KC582890.1.1797_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_alveolate
## 78 FN263031.1.1789_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_alveolate
## 4 KC488407.1.1693_Eukaryota;SAR;Alveolata;Dinoflagellata;Dinophyceae;B7;uncultured_dinoflagellate
## 42 KC582930.1.1796_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_alveolate
## 61 KC488409.1.1674_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_dinoflagellate
## 69 EU793381.1.1670_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_syndiniales
## 100 <NA>
## 101 EU793678.1.1564_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_syndiniales
## 34 EU780589.1.1635_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 15 GQ365865.1.1537_Eukaryota;Incertae_Sedis;Telonema;uncultured_eukaryote
## 2 EU793418.1.1665_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_syndiniales
## 82 EU793392.1.1594_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_V;uncultured_syndiniales
## 99 EU793367.1.1601_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_syndiniales
## 93 KC582884.1.1799_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_alveolate
## 5 <NA>
## 29 JN832733.1.1713_Eukaryota;Cryptophyceae;Kathablepharidae;Leucocryptos;uncultured_eukaryote
## 98 JX840917.1.1201_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_dinoflagellate
## 26 KC582995.1.1780_Eukaryota;SAR;Stramenopiles;Ochrophyta;Chrysophyceae;E222;uncultured_stramenopile
## 30 KC582941.1.1793_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_alveolate
## 103 EU793840.1.1684_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_syndiniales
## 59 FJ832105.1.1591_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_marine_plankton
## 73 AY665031.1.1749_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 57 GU820026.1.1394_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_dinoflagellate
## 7 AY665056.1.1740_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 74 KC488460.1.1689_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_marine_alveolate
## 53 EF172960.1.1674_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_eukaryote
## 19 KC582893.1.1795_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_alveolate
## 105 EF172986.1.1644_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 22 KC582920.1.1797_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_alveolate
## 3 FJ832116.1.1590_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_marine_plankton
## 9 JX188356.1.1758_Eukaryota;SAR;Alveolata;SCM37C52;uncultured_eukaryote
## 106 JX188283.1.1756_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_eukaryote
## 95 JQ420086.1.1778_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_phytoplankton
## 81 <NA>
## 45 EF172986.1.1644_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 16 EU793930.1.1664_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_syndiniales
## 14 KC582930.1.1796_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_alveolate
## 80 JN811213.1.1672_Eukaryota;SAR;Rhizaria;Retaria;Acantharia;Arthracanthida;Acanthometra;Xiphacantha_alata
## 6 EU793688.1.1653_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_II;uncultured_syndiniales
## 28 AY665036.1.1748_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_eukaryote
## 71 EU793901.1.1663_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_I;uncultured_syndiniales
## 63 GU820421.1.1200_Eukaryota;SAR;Alveolata;Protalveolata;Syndiniales;Syndiniales_Group_V;uncultured_dinoflagellate
## 32 JQ782054.1.1813_Eukaryota;SAR;Stramenopiles;MAST-4;uncultured_stramenopile
## MAS_classif
## 79 GQ913047_MALV-I_Alveolata
## 40 EU793773_MALV-I_Alveolata
## 48 FJ537481_Dinophyceae_Alveolata
## 50 FJ431657_MALV-III_Alveolata
## 1 EU793855_Dinophyceae_Alveolata
## 17 EU793468_MALV-II_Alveolata
## 88 EU793854_MALV-II_Alveolata
## 64 EU780636_Dinophyceae_Alveolata
## 35 EU793623_Dinophyceae_Alveolata
## 68 EU793487_Dinophyceae_Alveolata
## 38 EU793472_MALV-V_Alveolata
## 65 EU793835_Dinophyceae_Alveolata
## 83 EU793397_MALV-I_Alveolata
## 76 JQ781940_MAST-1D_Stramenopiles
## 58 FJ537481_Dinophyceae_Alveolata
## 89 JQ782036_MAST-1C_Stramenopiles
## 13 GQ913054_MALV-I_Alveolata
## 24 EU793906_MALV-II_Alveolata
## 96 FJ537505_MALV-II_Alveolata
## 23 EU793270_MALV-I_Alveolata
## 8 GQ382836_MALV-III_Alveolata
## 46 EU793869_MALV-II_Alveolata
## 97 EF172961_MAST-3A_Stramenopiles
## 62 EU561942_Dinophyceae_Alveolata
## 55 GQ382841_Dinophyceae_Alveolata
## 90 EU793665_MALV-I_Alveolata
## 84 EU793454_MALV-II_Alveolata
## 70 AY426916_Dinophyceae_Alveolata
## 52 ZZ0026100_Picobiliphyta_Hacrobia
## 94 EU818104_Dinophyceae_Alveolata
## 102 EU793515_MALV-III_Alveolata
## 18 EU561774_Dinophyceae_Alveolata
## 12 EU793725_Dinophyceae_Alveolata
## 92 DQ917937_MALV-III_Alveolata
## 85 EU793310_Dinophyceae_Alveolata
## 86 EU500204_Dinophyceae_Alveolata
## 21 EF539143_Picobiliphyta_Hacrobia
## 44 EU793423_MALV-I_Alveolata
## 51 EU793876_MALV-I_Alveolata
## 72 JQ782043_MAST-4A_Stramenopiles
## 87 EU817904_MALV-I_Alveolata
## 37 EU780618_Dinophyceae_Alveolata
## 47 AJ965134_MALV-II_Alveolata
## 25 AB241.O08_Dictyochophytes_Stramenopiles
## 20 EF173010_MAST-1D_Stramenopiles
## 56 AY938154_Dinophyceae_Alveolata
## 31 AY426873_MALV-I_Alveolata
## 49 EU793161_MALV-I_Alveolata
## 77 EU793591_MALV-II_Alveolata
## 66 EU562105_MALV-V_Alveolata
## 60 EU793849_MALV-I_Alveolata
## 39 GQ382521_MALV-I_Alveolata
## 36 EU793623_Dinophyceae_Alveolata
## 41 EU561962_MALV-I_Alveolata
## 54 AY129057_MALV-II_Alveolata
## 67 EU500119_Centroheliozoa_Hacrobia
## 75 EU793359_MALV-I_Alveolata
## 10 EU780632_Dinophyceae_Alveolata
## 11 EF526792_Dinophyceae_Alveolata
## 104 EU561765_MALV-I_Alveolata
## 43 GQ383169_MALV-II_Alveolata
## 33 EU793396_MALV-II_Alveolata
## 27 EU793496_MALV-II_Alveolata
## 91 EU793160_MALV-II_Alveolata
## 78 GQ382775_MALV-I_Alveolata
## 4 AY426916_Dinophyceae_Alveolata
## 42 EU562164_MALV-II_Alveolata
## 61 EU818099_MALV-I_Alveolata
## 69 EU793900_MALV-II_Alveolata
## 100 <NA>
## 101 EU818216_MALV-II_Alveolata
## 34 DQ918674_MALV-I_Alveolata
## 15 DQ119999_Telonemia_Hacrobia
## 2 EU818180_MALV-II_Alveolata
## 82 EU793880_MALV-V_Alveolata
## 99 EU793151_MALV-II_Alveolata
## 93 EU817983_MALV-II_Alveolata
## 5 EU500081_MAST-25_Stramenopiles
## 29 EU561799_Katablepharidales_Hacrobia
## 98 FJ537409_MALV-II_Alveolata
## 26 EF172998_Chrysophytes_Stramenopiles
## 30 FJ537539_MALV-II_Alveolata
## 103 EU793592_MALV-II_Alveolata
## 59 EU793662_MALV-II_Alveolata
## 73 EU793570_MALV-I_Alveolata
## 57 FJ537453_MALV-II_Alveolata
## 7 EU818437_MALV-I_Alveolata
## 74 AY665057_MALV-I_Alveolata
## 53 EU793316_MALV-II_Alveolata
## 19 EU793353_MALV-II_Alveolata
## 105 EU793360_MALV-I_Alveolata
## 22 EU793410_MALV-II_Alveolata
## 3 EU793860_MALV-II_Alveolata
## 9 EU561885_Dinophyceae_Alveolata
## 106 EU817986_MALV-II_Alveolata
## 95 EU561991_MALV-I_Alveolata
## 81 EU818263_MALV-II_Alveolata
## 45 EU793161_MALV-I_Alveolata
## 16 EU793468_MALV-II_Alveolata
## 14 AY937485_MALV-II_Alveolata
## 80 GQ913026_Acantharea_Rhizaria
## 6 EU793411_MALV-II_Alveolata
## 28 EU793607_MALV-I_Alveolata
## 71 EU818388_MALV-I_Alveolata
## 63 EU793284_MALV-V_Alveolata
## 32 HQ869203_MAST-4E_Stramenopiles
## BM_classif
## 79 32_MALV-I_Alveolata_15673
## 40 3261_MALV-I_Alveolata_385
## 48 5651_Dinophyceae_Alveolata_249
## 50 38_MALV-III_Alveolata_6570
## 1 9492_Dinophyceae_Alveolata_15
## 17 159_MALV-II_Alveolata_1129
## 88 549_MALV-II_Alveolata_410
## 64 21474_Dinophyceae_Alveolata_6
## 35 16659_Dinophyceae_Alveolata_16
## 68 19832_Dinophyceae_Alveolata_6
## 38 269_MALV-V_Alveolata_430
## 65 1849_Dinophyceae_Alveolata_250
## 83 10705_MALV-I_Alveolata_87
## 76 135_MAST-1_Stramenopiles_1096
## 58 5651_Dinophyceae_Alveolata_249
## 89 129_MAST-1_Stramenopiles_1402
## 13 10705_MALV-I_Alveolata_87
## 24 329_MALV-II_Alveolata_831
## 96 173_MALV-II_Alveolata_516
## 23 110_MALV-I_Alveolata_871
## 8 70_MALV-III_Alveolata_4778
## 46 2230_MALV-II_Alveolata_177
## 97 5352_MAST-3_Stramenopiles_16
## 62 60_Dinophyceae_Alveolata_2847
## 55 5854_Dinophyceae_Alveolata_118
## 90 2689_MALV-I_Alveolata_92
## 84 11869_MALV-II_Alveolata_32
## 70 63_Dinophyceae_Alveolata_4010
## 52 96_Picozoa_Hacrobia_1708
## 94 181_Dinophyceae_Alveolata_934
## 102 373_MALV-III_Alveolata_220
## 18 2659_Dinophyceae_Alveolata_438
## 12 12262_Dinophyceae_Alveolata_20
## 92 92_MALV-III_Alveolata_2457
## 85 250_Dinophyceae_Alveolata_874
## 86 9883_Dinophyceae_Alveolata_11
## 21 64_Picozoa_Hacrobia_4926
## 44 75_MALV-I_Alveolata_3817
## 51 90_MALV-I_Alveolata_1447
## 72 127_MAST-4_Stramenopiles_1171
## 87 10506_MALV-I_Alveolata_3
## 37 6794_Dinophyceae_Alveolata_12
## 47 449_MALV-II_Alveolata_595
## 25 1696_Dictyochophyceae_Stramenopiles_41
## 20 5093_MAST-1_Stramenopiles_47
## 56 9_Dinophyceae_Alveolata_61014
## 31 3261_MALV-I_Alveolata_385
## 49 12073_MALV-I_Alveolata_80
## 77 159_MALV-II_Alveolata_1129
## 66 269_MALV-V_Alveolata_430
## 60 320_MALV-I_Alveolata_280
## 39 4958_MALV-I_Alveolata_259
## 36 13136_Dinophyceae_Alveolata_79
## 41 408_MALV-I_Alveolata_437
## 54 372_MALV-II_Alveolata_174
## 67 228_Centrohelida_Hacrobia_542
## 75 110_MALV-I_Alveolata_871
## 10 15157_Dinophyceae_Alveolata_44
## 11 4619_Dinophyceae_Alveolata_56
## 104 274_MALV-I_Alveolata_291
## 43 431_MALV-II_Alveolata_283
## 33 538_MALV-II_Alveolata_49
## 27 396_MALV-II_Alveolata_141
## 91 6845_MALV-II_Alveolata_162
## 78 5250_MALV-I_Alveolata_344
## 4 63_Dinophyceae_Alveolata_4010
## 42 437_MALV-II_Alveolata_11
## 61 161_MALV-I_Alveolata_1735
## 69 666_MALV-II_Alveolata_7
## 100 512_Dictyochophyceae_Stramenopiles_107
## 101 183_MALV-II_Alveolata_589
## 34 979_MALV-I_Alveolata_83
## 15 201_Telonema_Hacrobia_877
## 2 437_MALV-II_Alveolata_11
## 82 269_MALV-V_Alveolata_430
## 99 143_MALV-II_Alveolata_365
## 93 1768_MALV-II_Alveolata_24
## 5 470_MAST-25_Stramenopiles_101
## 29 299_Katablepharidae_Hacrobia_783
## 98 666_MALV-II_Alveolata_7
## 26 524_Chrysophyceae_Stramenopiles_220
## 30 15297_MALV-II_Alveolata_17
## 103 13359_MALV-II_Alveolata_15
## 59 234_MALV-II_Alveolata_626
## 73 195_MALV-I_Alveolata_265
## 57 779_MALV-II_Alveolata_39
## 7 90_MALV-I_Alveolata_1447
## 74 152_MALV-I_Alveolata_1517
## 53 <NA>
## 19 1019_MALV-II_Alveolata_76
## 105 9794_MALV-I_Alveolata_9
## 22 1019_MALV-II_Alveolata_76
## 3 12035_MALV-II_Alveolata_25
## 9 3063_Dinophyceae_Alveolata_28
## 106 173_MALV-II_Alveolata_516
## 95 75_MALV-I_Alveolata_3817
## 81 698_MALV-II_Alveolata_96
## 45 12073_MALV-I_Alveolata_80
## 16 159_MALV-II_Alveolata_1129
## 14 437_MALV-II_Alveolata_11
## 80 46_Acantharia_Rhizaria_1903
## 6 183_MALV-II_Alveolata_589
## 28 320_MALV-I_Alveolata_280
## 71 398_MALV-I_Alveolata_395
## 63 269_MALV-V_Alveolata_430
## 32 256_MAST-4_Stramenopiles_625Number and proportion (%) of cosmopolitan OTUs:
## [1] 106## [1] 0.2764881Number and proportion (%) of rare OTUs:
## [1] 32638## [1] 85.132241.6) Taxonomic composition analysis
1.6.1) Normalized data
No. of OTUs and reads of the rearefied dataset:
## [1] 38338## [1] 4547725
Number and proportion (%) of OTUs of phototrophic groups:
## [1] 11814## [1] 30.81538Number and proportion (%) of reads of phototrophic groups:
## [1] 950083## [1] 20.89139
Number and proportion (%) of OTUs of non-phototrophic groups:
## [1] 26524## [1] 69.18462Number and proportion (%) of reads of non-phototrophic groups:
## [1] 3597642## [1] 79.10861
PHOTOTROPHS + HETEROTROPHS
Absolute values
## reads_per_class OTUs_per_class samples_per_class
## Bacillariophyceae 3654 101 89
## Bolidophyceae 2869 16 89
## Chlorarachniophyceae 3397 68 87
## Chrysophyceae 79402 357 91
## Cryptophyceae 9897 75 78
## Dictyochophyceae 39365 239 91
## Dinophyceae 650163 9852 91
## Eustigmatophyceae 192 9 24
## Heterotrophs 3597642 26524 91
## MOCH_1 4367 76 88
## MOCH_2 16896 135 91
## MOCH_5 2400 22 74
## Mamiellophyceae 321 33 45
## Pelagophyceae 47197 333 90
## Pinguiophyceae 1551 20 88
## Prasinophyceae_clade-IX 11939 83 86
## Prasinophyceae_clade-VII 24093 123 88
## Prymnesiophyceae 387 58 77
## Pyramimonadaceae 113 3 7
## Raphydophyceae 214 4 51
## Trebouxiophyceae 102 3 4
## Ulvophyceae 11 3 3
## other_Prasinophyceae 51553 201 91
Relative values
## reads_per_class OTUs_per_class samples_per_class
## 100.000 100.000 1773.626## reads_per_class OTUs_per_class samples_per_class
## Bacillariophyceae 8.034787e-02 0.263446189 97.802198
## Bolidophyceae 6.308649e-02 0.041734050 97.802198
## Chlorarachniophyceae 7.469669e-02 0.177369712 95.604396
## Chrysophyceae 1.745972e+00 0.931190985 100.000000
## Cryptophyceae 2.176253e-01 0.195628358 85.714286
## Dictyochophyceae 8.655976e-01 0.623402368 100.000000
## Dinophyceae 1.429644e+01 25.697741145 100.000000
## Eustigmatophyceae 4.221891e-03 0.023475403 26.373626
## Heterotrophs 7.910861e+01 69.184621003 100.000000
## MOCH_1 9.602603e-02 0.198236736 96.703297
## MOCH_2 3.715264e-01 0.352131045 100.000000
## MOCH_5 5.277364e-02 0.057384318 81.318681
## Mamiellophyceae 7.058474e-03 0.086076478 49.450549
## Pelagophyceae 1.037816e+00 0.868589911 98.901099
## Pinguiophyceae 3.410496e-02 0.052167562 96.703297
## Prasinophyceae_clade-IX 2.625269e-01 0.216495383 94.505495
## Prasinophyceae_clade-VII 5.297814e-01 0.320830508 96.703297
## Prymnesiophyceae 8.509749e-03 0.151285930 84.615385
## Pyramimonadaceae 2.484759e-03 0.007825134 7.692308
## Raphydophyceae 4.705650e-03 0.010433512 56.043956
## Trebouxiophyceae 2.242880e-03 0.007825134 4.395604
## Ulvophyceae 2.418792e-04 0.007825134 3.296703
## other_Prasinophyceae 1.133600e+00 0.524284000 100.000000
Reads per class vs. OTUs per class:
Reads per class vs. samples in which they occurr:
PHOTOTROPHS
Absolute values
## reads_per_class OTUs_per_class samples_per_class
## Bacillariophyceae 3654 101 89
## Bolidophyceae 2869 16 89
## Chlorarachniophyceae 3397 68 87
## Chrysophyceae 79402 357 91
## Cryptophyceae 9897 75 78
## Dictyochophyceae 39365 239 91
## Dinophyceae 650163 9852 91
## Eustigmatophyceae 192 9 24
## MOCH_1 4367 76 88
## MOCH_2 16896 135 91
## MOCH_5 2400 22 74
## Mamiellophyceae 321 33 45
## Pelagophyceae 47197 333 90
## Pinguiophyceae 1551 20 88
## Prasinophyceae_clade-IX 11939 83 86
## Prasinophyceae_clade-VII 24093 123 88
## Prymnesiophyceae 387 58 77
## Pyramimonadaceae 113 3 7
## Raphydophyceae 214 4 51
## Trebouxiophyceae 102 3 4
## Ulvophyceae 11 3 3
## other_Prasinophyceae 51553 201 91
Relative values
## reads_per_class OTUs_per_class samples_per_class
## 100.000 100.000 1673.626## reads_per_class OTUs_per_class samples_per_class
## Bacillariophyceae 0.384597977 0.85491789 97.802198
## Bolidophyceae 0.301973617 0.13543254 97.802198
## Chlorarachniophyceae 0.357547709 0.57558829 95.604396
## Chrysophyceae 8.357375092 3.02183850 100.000000
## Cryptophyceae 1.041698462 0.63484002 85.714286
## Dictyochophyceae 4.143322215 2.02302353 100.000000
## Dinophyceae 68.432231710 83.39258507 100.000000
## Eustigmatophyceae 0.020208761 0.07618080 26.373626
## MOCH_1 0.459644052 0.64330455 96.703297
## MOCH_2 1.778370942 1.14271204 100.000000
## MOCH_5 0.252609509 0.18621974 81.318681
## Mamiellophyceae 0.033786522 0.27932961 49.450549
## Pelagophyceae 4.967671246 2.81868969 98.901099
## Pinguiophyceae 0.163248895 0.16929067 96.703297
## Prasinophyceae_clade-IX 1.256627053 0.70255629 94.505495
## Prasinophyceae_clade-VII 2.535883707 1.04113763 96.703297
## Prymnesiophyceae 0.040733283 0.49094295 84.615385
## Pyramimonadaceae 0.011893698 0.02539360 7.692308
## Raphydophyceae 0.022524348 0.03385813 56.043956
## Trebouxiophyceae 0.010735904 0.02539360 4.395604
## Ulvophyceae 0.001157794 0.02539360 3.296703
## other_Prasinophyceae 5.426157504 1.70137125 100.000000
Reads per class vs. OTUs per class:
Reads per class vs. samples in which they occurr:
1.6.2) Non-rarefied data
No. of OTUs and reads of the rearefied dataset:
## [1] 43734## [1] 12250242
Number and proportion (%) of OTUs of phototrophic groups:
## [1] 13554## [1] 30.99191Number and proportion (%) of reads of phototrophic groups:
## [1] 1224986## [1] 21.25556
Number and proportion (%) of OTUs of non-phototrophic groups:
## [1] 30180## [1] 69.00809Number and proportion (%) of reads of non-phototrophic groups:
## [1] 4538145## [1] 78.74444
PHOTOTROPHS + HETEROTROPHS
Absolute values
## reads_per_class OTUs_per_class samples_per_class
## Bacillariophyceae 9195 109 89
## Bolidophyceae 7156 16 89
## Chlorarachniophyceae 10036 80 89
## Chrysophyceae 192835 418 91
## Cryptophyceae 27260 95 81
## Dictyochophyceae 104205 264 91
## Dinophyceae 1769257 11319 91
## Eustigmatophyceae 573 9 31
## Heterotrophs 9735284 30180 91
## MOCH_1 13716 83 88
## MOCH_2 45703 153 91
## MOCH_5 7015 25 75
## Mamiellophyceae 931 35 48
## Pelagophyceae 121087 386 90
## Pinguiophyceae 5296 27 89
## Prasinophyceae_clade-IX 29715 86 87
## Prasinophyceae_clade-VII 56610 133 88
## Prymnesiophyceae 1072 74 80
## Pyramimonadaceae 254 3 7
## Raphydophyceae 664 4 62
## Trebouxiophyceae 193 3 4
## Ulvophyceae 50 3 5
## other_Prasinophyceae 112135 229 91
Relative values
## reads_per_class OTUs_per_class samples_per_class
## 100.000 100.000 1810.989## reads_per_class OTUs_per_class samples_per_class
## Bacillariophyceae 7.505974e-02 0.249234006 97.802198
## Bolidophyceae 5.841517e-02 0.036584808 97.802198
## Chlorarachniophyceae 8.192491e-02 0.182924041 97.802198
## Chrysophyceae 1.574132e+00 0.955778113 100.000000
## Cryptophyceae 2.225262e-01 0.217222298 89.010989
## Dictyochophyceae 8.506363e-01 0.603649335 100.000000
## Dinophyceae 1.444263e+01 25.881465222 100.000000
## Eustigmatophyceae 4.677459e-03 0.020578955 34.065934
## Heterotrophs 7.947014e+01 69.008094389 100.000000
## MOCH_1 1.119651e-01 0.189783692 96.703297
## MOCH_2 3.730783e-01 0.349842228 100.000000
## MOCH_5 5.726417e-02 0.057163763 82.417582
## Mamiellophyceae 7.599850e-03 0.080029268 52.747253
## Pelagophyceae 9.884458e-01 0.882608497 98.901099
## Pinguiophyceae 4.323180e-02 0.061736864 97.802198
## Prasinophyceae_clade-IX 2.425666e-01 0.196643344 95.604396
## Prasinophyceae_clade-VII 4.621133e-01 0.304111218 96.703297
## Prymnesiophyceae 8.750848e-03 0.169204738 87.912088
## Pyramimonadaceae 2.073428e-03 0.006859652 7.692308
## Raphydophyceae 5.420301e-03 0.009146202 68.131868
## Trebouxiophyceae 1.575479e-03 0.006859652 4.395604
## Ulvophyceae 4.081552e-04 0.006859652 5.494505
## other_Prasinophyceae 9.153697e-01 0.523620067 100.000000
Reads per class vs. OTUs per class:
Reads per class vs. samples in which they occurr:
PHOTOTROPHS
Absolute values
## reads_per_class OTUs_per_class samples_per_class
## Bacillariophyceae 9195 109 89
## Bolidophyceae 7156 16 89
## Chlorarachniophyceae 10036 80 89
## Chrysophyceae 192835 418 91
## Cryptophyceae 27260 95 81
## Dictyochophyceae 104205 264 91
## Dinophyceae 1769257 11319 91
## Eustigmatophyceae 573 9 31
## MOCH_1 13716 83 88
## MOCH_2 45703 153 91
## MOCH_5 7015 25 75
## Mamiellophyceae 931 35 48
## Pelagophyceae 121087 386 90
## Pinguiophyceae 5296 27 89
## Prasinophyceae_clade-IX 29715 86 87
## Prasinophyceae_clade-VII 56610 133 88
## Prymnesiophyceae 1072 74 80
## Pyramimonadaceae 254 3 7
## Raphydophyceae 664 4 62
## Trebouxiophyceae 193 3 4
## Ulvophyceae 50 3 5
## other_Prasinophyceae 112135 229 91
Relative values
## reads_per_class OTUs_per_class samples_per_class
## 100.000 100.000 1710.989## reads_per_class OTUs_per_class samples_per_class
## Bacillariophyceae 0.365612467 0.80419064 97.802198
## Bolidophyceae 0.284537555 0.11804633 97.802198
## Chlorarachniophyceae 0.399052390 0.59023167 97.802198
## Chrysophyceae 7.667523672 3.08396045 100.000000
## Cryptophyceae 1.083914721 0.70090010 89.010989
## Dictyochophyceae 4.143409154 1.94776450 100.000000
## Dinophyceae 70.349365675 83.51040283 100.000000
## Eustigmatophyceae 0.022783681 0.06640106 34.065934
## MOCH_1 0.545376901 0.61236535 96.703297
## MOCH_2 1.817247047 1.12881806 100.000000
## MOCH_5 0.278931099 0.18444740 82.417582
## Mamiellophyceae 0.037018511 0.25822635 52.747253
## Pelagophyceae 4.814672849 2.84786779 98.901099
## Pinguiophyceae 0.210580057 0.19920319 97.802198
## Prasinophyceae_clade-IX 1.181530666 0.63449904 95.604396
## Prasinophyceae_clade-VII 2.250932222 0.98126014 96.703297
## Prymnesiophyceae 0.042624966 0.54596429 87.912088
## Pyramimonadaceae 0.010099572 0.02213369 7.692308
## Raphydophyceae 0.026402031 0.02951158 68.131868
## Trebouxiophyceae 0.007674084 0.02213369 4.395604
## Ulvophyceae 0.001988105 0.02213369 5.494505
## other_Prasinophyceae 4.458722571 1.68953814 100.000000
Reads per class vs. OTUs per class:
Reads per class vs. samples in which they occurr:
